README for Figure S4.csv 
*** This file contains the raw data obtained on DNA and DNA-Rok complexes using bridging assay experiments represented in Figure S4 of 
Article: The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues
Authors: Erkelens, Qin, van Erp, Miguel-Arribas, Abia, Keek, Markus, Cajili, Schwab, Meijer and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl; wmeijer@cbm.csic.es


Legend Figure S4:1,6-hexanediol cannot prevent Rok-DNA bridges being formed DNA recovery (%) as a function of 
added 1,6-hexanediol (%) in the presence of 0.27 M Rok with 50 mM KCl at 25C. Data are plotted as mean values 
and the error bars represent the standard deviation from three independent measurements.

*** The data were obtained using bridging assay experiments as described in the associated article. 

***The data labeled overview represents the values plotted in the graph of figure S4. The raw data contains the individual replicates.
Column A: Sample number
Column B: Presence of bait DNA in sample
Column C: Presence of 32P-labeled prey DNA in sample
Column D: Amount of 1,6 hexanediol in sample (in %)
Column E: Counts per minutes 
Column F: DNA recovery (%)

Columns B, C and D indicate the contents of the sample. Column E indicates the radioactivity of the sample. 
Column F indicates the DNA recovery, which is calculated by subtracting the value in column F of the control 
(sample minus bait DNA) from the sample itself. This value is then divided by the values in column E of sample 1 
and multiplied by 100%. 









